top of page

Publications 研究発表

発表論文

・Kimura T, Yamashita A, Ozono K, Tsumaki N.

 Limited Immunogenicity of Human Induced Pluripotent Stem Cell-Derived Cartilages.

 Tissue Eng Part A. 2016 Dec;22(23-24):1367-1375. Epub 2016 Oct 31.

 

・Yasuhito Yahara, Hiroshi Takemori, Minoru Okada, Azuma Kosai, Akihiro Yamashita, Tomohito  

 Kobayashi, Kaori Fujita, Yumi Itoh, Masahiro Nakamura, Hiroyuki Fuchino, Nobuo Kawahara,

 Naoshi Fukui, Akira Watanabe, Tomoatsu Kimura and Noriyuki Tsumaki

 Pterosin B prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting Sik3

 Nature Communications, 2016; 7:10959

・Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S.,

 and Tsumaki, N.

 Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs.

 Stem cell reports, 2015; 4: 404-418.

・Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H,

 Okamoto T, Nishimura G, Imaizumi K, Tsumaki N.

 Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced

 pluripotent stem cells.

 Hum Mol Genet, 2015; 24: 299-313.

・Yamashita A, Morioka M, Kishi H, Kimura T, Yahara Y, Okada M, Fujita K, Sawai H, Ikegawa S,

 Tsumaki N.

 Statin treatment rescues FGFR3 skeletal dysplasia phenotypes.

 Nature, 2014; 513: 507-511.

・Tam WL, Dorien FO, Hiramatsu K, Tsumaki N, Luyten FP, Roberts SJ.

 Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro

 and trigger endochondral ossification in vivo.

 Cell Reprogram, 2014; 16: 29-39.

・Seki S, Tsumaki N, Motomura H, Nogami M, Kawaguchi Y, Hori T, Suzuki K, Yahara Y,

 Higashimoto M, Oya T, Ikegawa S, Kimura T.

 Cartilage intermediate layer protein promotes lumbar disc degeneration.

 Biochem Biophys Res Commun, 2014; 446: 876-881.

・Minegishi Y, Sakai Y, Yahara Y, Akiyama H, Yoshikawa H, Hosokawa K, Tsumaki N.

 Cyp26b1 within the growth plate regulates bone growth in juvenile mice.

 Biochem Biophys Res Commun, 2014; 454: 12-18.

・Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N.

 Direct induction of chondrogenic cells from human dermal fibroblast culture by defined   

 factors.

 PLoS One, 2013; 8: e77365.

 

・Minegishi Y, Hosokawa K, Tsumaki N.

 Time-lapse observation of the dedifferentiation process in mouse chondrocytes using

 chondrocyte-specific reporters.

 Osteoarthritis Cartilage, 2013; 21: 1968-1975.

・Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K,

 Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T,

 Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, Kawahara H,

 Tomonaga T, Naka T, Ikegawa S, Tsumaki N, Matsuda J, Takemori H.

 Involvement of SIK3 in glucose and lipid homeostasis in mice.

 PLoS One, 2012; 7: e37803.

・Sasagawa S, Takemori H, Uebi T, Ikegami D, Hiramatsu K, Ikegawa S, Yoshikawa H, Tsumaki N.

 SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice.

 Development, 2012; 139: 1153-1163.

・Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G,

 Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T.

 Osterix regulates calcification and degradation of chondrogenic matrices through matrix

 metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2

 during endochondral ossification.

 J Biol Chem, 2012; 287: 33179-33190.

・Miura K, Namba N, Fujiwara M, Ohata Y, Ishida H, Kitaoka T, Kubota T, Hirai H, Higuchi C,

 Tsumaki N, Yoshikawa H, Sakai N, Michigami T, Ozono K.

 An Overgrowth Disorder Associated with Excessive Production of cGMP Due to a

 Gain-of-Function Mutation of the Natriuretic Peptide Receptor 2 Gene.

 PLoS One, 2012; 7: e42180.

・Hiramatsu, K., Sasagawa, S., Outani, H., Nakagawa, K., Yoshikawa, H. and Tsumaki, N.

 Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture

 by defined factors.

 J. Clin. Invest., 121: 640-57, 2011.

・Ikegami, D., Akiyama, H., Suzuki, A., Nakamura, T., Nakano, T., Yoshikawa, H. and Tsumaki, N

 Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways.

 Development, 138: 1507-19, 2011.

・Okamoto, M., Murai, J., Imai, Y., Ikegami, D., Kamiya, N., Kato, S., Mishina, Y., Yoshikawa, H.

 and Tsumaki, N

 Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone

 formation, increasing volume of remodeling bone in mice.

 J. Bone Miner. Res., 26: 2511-22, 2011.

・Ikegami, D., Iwai, T., Ryo, S., Gu, N., Sugiyama, T., Oh, I., Yoshikawa, H. and Tsumaki, N

 Identification of small molecular compounds and fabrication of its aqueous solution by  

 laser-ablation, expanding primordial cartilage

 Osteoarthritis Cartilage, 19: 233-41, 2011.

・Outani, H., Okada, M., Hiramatsu, K., Yoshikawa, H. and Tsumaki, N

 Induction of chondrogenic cells from dermal fibroblast culture by defined factors does not  

 involve a pluripotent state.

 Biochem. Biophys. Res. Commun., 411: 607-12, 2011.

・Hiramatsu, K., Iwai, T., Yoshikawa, H. and Tsumaki, N

 Expression of dominant negative TGF-beta receptors inhibits cartilage formation in

 conditional transgenic mice.

 J. Bone Miner. Metab., 29: 493-500, 2011.

・Nakamura, Y., Yamamoto, K., He, X., Otsuki, B., Kim, Y., Murao, H., Soeda, T., Tsumaki, N.,

 Deng, J. M., Zhang, Z., Behringer, R. R., Crombrugghe, B., Postlethwait, J. H., Warman, M. L.,

 Nakamura, T. and Akiyama, H

 Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and

 mediator subunit 25

 Nat Commun, 2: 251, 2011.

・Suzuki, D., Yamada, A., Amano, T., Yasuhara, R., Kimura, A., Sakahara, M., Tsumaki, N.,

 Takeda, S., Tamura, M., Nakamura, M., Wada, N., Nohno, T., Shiroishi, T., Aiba, A. and Kamijo, R

 Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death

 during limb development.

 Dev. Biol., 335: 396-406, 2009.

・Iwai, T., Murai, J., Yoshikawa, H. and Tsumaki, N.

 Smad7 Inhibits chondrocyte differentiation at multiple steps during endochondral bone

 formation and down-regulates p38 MAPK pathways

 J. Biol. Chem., 283: 27154-64, 2008.

・Murai, J., Ikegami, D., Okamoto, M., Yoshikawa, H. and Tsumaki, N

 Insulation of the ubiquitous Rxrb promoter from the cartilage-specific adjacent gene,

 Col11a2

 J. Biol. Chem., 283: 27677-87, 2008.

・Nampei, A., Hashimoto, J., Koyanagi, J., Ono, T., Hashimoto, H., Tsumaki, N., Tomita, T.,

 Sugamoto, K., Nishimoto, N., Ochi, T. and Yoshikawa, H.:

 Characteristics of fracture and related factors in patients with rheumatoid arthritis.

 Modern rheumatology / the Japan Rheumatism Association, 18: 170-6, 2008.

・Sugiki, T., Uyama, T., Toyoda, M., Morioka, H., Kume, S., Miyado, K., Matsumoto, K., Saito, H.,

 Tsumaki, N., Takahashi, Y., Toyama, Y. and Umezawa, A.

 Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9  

 chondroblasts.

 J. Cell. Biochem., 100: 1240-54, 2007.

・Iwai, T., Harada, Y., Imura, K., Iwabuchi, S., Murai, J., Hiramatsu, K., Myoui, A., Yoshikawa, H.

 and Tsumaki, N.

 Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite

 ceramicJ.

 Bone Miner. Metab., 25: 392-9, 2007.

・Okamoto, M., Murai, J., Yoshikawa, H. and Tsumaki, N

 Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts during bone

 development.J.

 Bone Miner. Res., 21: 1022-33, 2006.

・Hirao, M., Tamai, N., Tsumaki, N., Yoshikawa, H. and Myoui, A

 Oxygen tension regulates chondrocyte differentiation and function during endochondral

 ossification.

 J. Biol. Chem., 281: 31079-92, 2006.

・Tsumaki, N., Kakiuchi, M., Sasaki, J., Ochi, T. and Yoshikawa, H

 Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with

 opening-wedge high tibial osteotomy by hemicallotasis

 J. Bone Joint Surg. Am., 86-A: 2399-405, 2004.

・Horiki, M., Imamura, T., Okamoto, M., Hayashi, M., Murai, J., Myoui, A., Ochi, T., Miyazono, K.,

 Yoshikawa, H. and Tsumaki, N

 Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes

 dwarfism with osteopenia.

 J. Cell Biol., 165: 433-45, 2004.

・Katayama, R., Wakitani, S., Tsumaki, N., Morita, Y., Matsushita, I., Gejo, R. and Kimura, T

 Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous

 mesenchymal cells derived from bone marrow

 Rheumatology (Oxford). 43: 980-5, 2004.

・Matsui, Y., Chansky, H. A., Barahmand-Pour, F., Zielinska-Kwiatkowska, A., Tsumaki, N.,

 Myoui, A., Yoshikawa, H., Yang, L. and Eyre, D. R

 COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma

 fusion protein and wild-type ERG

 J. Biol. Chem., 278: 11369-75, 2003

・Tsumaki, N., Nakase, T., Miyaji, T., Kakiuchi, M., Kimura, T., Ochi, T. and Yoshikawa, H

 Bone morphogenetic protein signals are required for cartilage formation and differently

 regulate joint development during skeletogenesis

 J. Bone Miner. Res., 17: 898-906, 2002.

・Nakase, T., Sugamoto, K., Miyamoto, T., Tsumaki, N., Luyten, F. P., Inui, H., Myoui, A., Tomita, T.  

 and Yoshikawa, H

 Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff.

 Clin Orthop Relat Res: 140-5, 2002

・Matsui, Y., Araki, N., Tsuboi, H., Tsumaki, N., Nakata, K., Kawabata, H., Eyre, D. R. and

 Yoshikawa, H

 Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells

 Clin Orthop Relat Res: 140-5, 2002

・Nakase, T., Ariga, K., Yonenobu, K., Tsumaki, N., Luyten, F. P., Mukai, Y., Sato, I. and

 Yoshikawa, H

 Activation and localization of cartilage-derived morphogenetic protein-1 at the site of

 ossification of the ligamentum flavum

 Eur. Spine J., 10: 289-94, 2001.

・Matsui, Y., Nakata, K., Araki, N., Ozono, K., Fujita, Y., Tsumaki, N., Kawabata, H., Yasui, N.,

 Sekiguchi, K. and Yoshikawa, H

 Alternative splicing of fibronectin transcripts in osteochondrogenic tumors

 Anticancer Res., 21: 1103-6, 2001

・Matsui, Y., Yasui, N., Kawabata, H., Ozono, K., Nakata, K., Mizushima, T., Tsumaki, N.,

 Kataoka, E., Fujita, Y. and Ochi, T

 A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal

 chondrodysplasia.

 J. Hum. Genet., 45: 105-8, 2000.

・Liu, Y., Li, H., Tanaka, K., Tsumaki, N. and Yamada, Y.

 Identification of an enhancer sequence within the first intron required for cartilage-specific  

 transcription of the alpha2(XI) collagen gene

 J. Biol. Chem., 275: 12712-8, 2000.

・Tsumaki, N., Tanaka, K., Arikawa-Hirasawa, E., Nakase, T., Kimura, T., Thomas, J. T., Ochi, T.,

 Luyten, F. P. and Yamada, Y.

 Role of CDMP-1 in skeletal morphogenesis: promotion of mesenchymal cell recruitment

 and chondrocyte differentiation.

 J. Cell Biol., 144: 161-73, 1999

 

・Tsumaki, N., Kimura, T., Tanaka, K., Kimura, J. H., Ochi, T. and Yamada, Y.

 Modular arrangement of cartilage- and neural tissue-specific cis-elements in the mouse

 alpha2(XI) collagen promoter.

 J. Biol. Chem., 273: 22861-4, 1998.

・Sugimoto, M., Kimura, T., Tsumaki, N., Matsui, Y., Nakata, K., Kawahata, H., Yasui, N.,

 Kitamura, Y., Nomura, S. and Ochi, T

 Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing

 mouse

 Cell Tissue Res., 292: 325-32, 1998.

・Matsui, Y., Yasui, N., Kimura, T., Tsumaki, N., Kawabata, H. and Ochi, T.

 Genotype phenotype correlation in achondroplasia and hypochondroplasia.

 J. Bone Joint Surg. Br., 80: 1052-6, 1998.

・Matsui, Y., Kimura, T., Tsumaki, N., Nakata, K., Yasui, N., Araki, N., Hashimoto, N., Uchida, A.

 and Ochi, T

 Splicing patterns of type XI collagen transcripts act as molecular markers for  

 osteochondrogenic tumors

 Cancer Lett., 124: 143-8, 1998.

・Tsumaki, N., Kimura, T., Matsui, Y., Nakata, K. and Ochi, T.

 Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI)

 collagen gene expression in the embryonic mouse cartilage.

 J. Cell Biol., 134: 1573-82, 1996.

・Tsumaki, N., Sugimoto, M., Nakata, K., Matsui, Y., Ochi, T. and Kimura, T.

 Separable regulatory elements from pro-alpha 2 (XI) collagen gene drive distinct patterns

 of cartilage-specific expression in transgenic mice.

 Ann. N. Y. Acad. Sci., 785: 340-2, 1996.

 

・Matsui, Y., Kimura, T., Tsumaki, N., Yasui, N. and Ochi, T.

 A recurrent 1992delCT mutation of the type X collagen gene in a Japanese patient with

 Schmid metaphyseal chondrodysplasia.

 Jpn. J. Hum. Genet., 41: 339-42, 1996.

・Kimura, T., Nakata, K., Tsumaki, N., Miyamoto, S., Matsui, Y., Ebara, S. and Ochi, T.

 Progressive degeneration of articular cartilage and intervertebral discs. An experimental  

 study in transgenic mice bearing a type IX collagen mutation

 Int. Orthop., 20: 177-81, 1996

・Iwasaki, M., Okada, K., Tsumaki, N., Obata, H., Shirasaki, N. and Oka, S

 Cervical spondylotic radiculopathy involving two adjacent nerve roots. Anterior

 decompression through a single level intervertebral approach.

 Int. Orthop., 20: 137-41, 1996.

・Tsumaki, N. and Kimura, T.

 Differential expression of an acidic domain in the amino-terminal propeptide of mouse

 pro-alpha 2(XI) collagen by complex alternative splicing.

 J. Biol. Chem., 270: 2372-8, 1995.

 

bottom of page