Publications 研究発表
発表論文
・Kimura T, Yamashita A, Ozono K, Tsumaki N.
Limited Immunogenicity of Human Induced Pluripotent Stem Cell-Derived Cartilages.
Tissue Eng Part A. 2016 Dec;22(23-24):1367-1375. Epub 2016 Oct 31.
・Yasuhito Yahara, Hiroshi Takemori, Minoru Okada, Azuma Kosai, Akihiro Yamashita, Tomohito
Kobayashi, Kaori Fujita, Yumi Itoh, Masahiro Nakamura, Hiroyuki Fuchino, Nobuo Kawahara,
Naoshi Fukui, Akira Watanabe, Tomoatsu Kimura and Noriyuki Tsumaki
Pterosin B prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting Sik3
Nature Communications, 2016; 7:10959
・Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S.,
and Tsumaki, N.
Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs.
Stem cell reports, 2015; 4: 404-418.
・Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H,
Okamoto T, Nishimura G, Imaizumi K, Tsumaki N.
Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced
pluripotent stem cells.
Hum Mol Genet, 2015; 24: 299-313.
・Yamashita A, Morioka M, Kishi H, Kimura T, Yahara Y, Okada M, Fujita K, Sawai H, Ikegawa S,
Tsumaki N.
Statin treatment rescues FGFR3 skeletal dysplasia phenotypes.
Nature, 2014; 513: 507-511.
・Tam WL, Dorien FO, Hiramatsu K, Tsumaki N, Luyten FP, Roberts SJ.
Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro
and trigger endochondral ossification in vivo.
Cell Reprogram, 2014; 16: 29-39.
・Seki S, Tsumaki N, Motomura H, Nogami M, Kawaguchi Y, Hori T, Suzuki K, Yahara Y,
Higashimoto M, Oya T, Ikegawa S, Kimura T.
Cartilage intermediate layer protein promotes lumbar disc degeneration.
Biochem Biophys Res Commun, 2014; 446: 876-881.
・Minegishi Y, Sakai Y, Yahara Y, Akiyama H, Yoshikawa H, Hosokawa K, Tsumaki N.
Cyp26b1 within the growth plate regulates bone growth in juvenile mice.
Biochem Biophys Res Commun, 2014; 454: 12-18.
・Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N.
Direct induction of chondrogenic cells from human dermal fibroblast culture by defined
factors.
PLoS One, 2013; 8: e77365.
・Minegishi Y, Hosokawa K, Tsumaki N.
Time-lapse observation of the dedifferentiation process in mouse chondrocytes using
chondrocyte-specific reporters.
Osteoarthritis Cartilage, 2013; 21: 1968-1975.
・Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K,
Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T,
Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, Kawahara H,
Tomonaga T, Naka T, Ikegawa S, Tsumaki N, Matsuda J, Takemori H.
Involvement of SIK3 in glucose and lipid homeostasis in mice.
PLoS One, 2012; 7: e37803.
・Sasagawa S, Takemori H, Uebi T, Ikegami D, Hiramatsu K, Ikegawa S, Yoshikawa H, Tsumaki N.
SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice.
Development, 2012; 139: 1153-1163.
・Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G,
Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T.
Osterix regulates calcification and degradation of chondrogenic matrices through matrix
metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2
during endochondral ossification.
J Biol Chem, 2012; 287: 33179-33190.
・Miura K, Namba N, Fujiwara M, Ohata Y, Ishida H, Kitaoka T, Kubota T, Hirai H, Higuchi C,
Tsumaki N, Yoshikawa H, Sakai N, Michigami T, Ozono K.
An Overgrowth Disorder Associated with Excessive Production of cGMP Due to a
Gain-of-Function Mutation of the Natriuretic Peptide Receptor 2 Gene.
PLoS One, 2012; 7: e42180.
・Hiramatsu, K., Sasagawa, S., Outani, H., Nakagawa, K., Yoshikawa, H. and Tsumaki, N.
Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture
by defined factors.
J. Clin. Invest., 121: 640-57, 2011.
・Ikegami, D., Akiyama, H., Suzuki, A., Nakamura, T., Nakano, T., Yoshikawa, H. and Tsumaki, N
Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways.
Development, 138: 1507-19, 2011.
・Okamoto, M., Murai, J., Imai, Y., Ikegami, D., Kamiya, N., Kato, S., Mishina, Y., Yoshikawa, H.
and Tsumaki, N
Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone
formation, increasing volume of remodeling bone in mice.
J. Bone Miner. Res., 26: 2511-22, 2011.
・Ikegami, D., Iwai, T., Ryo, S., Gu, N., Sugiyama, T., Oh, I., Yoshikawa, H. and Tsumaki, N
Identification of small molecular compounds and fabrication of its aqueous solution by
laser-ablation, expanding primordial cartilage
Osteoarthritis Cartilage, 19: 233-41, 2011.
・Outani, H., Okada, M., Hiramatsu, K., Yoshikawa, H. and Tsumaki, N
Induction of chondrogenic cells from dermal fibroblast culture by defined factors does not
involve a pluripotent state.
Biochem. Biophys. Res. Commun., 411: 607-12, 2011.
・Hiramatsu, K., Iwai, T., Yoshikawa, H. and Tsumaki, N
Expression of dominant negative TGF-beta receptors inhibits cartilage formation in
conditional transgenic mice.
J. Bone Miner. Metab., 29: 493-500, 2011.
・Nakamura, Y., Yamamoto, K., He, X., Otsuki, B., Kim, Y., Murao, H., Soeda, T., Tsumaki, N.,
Deng, J. M., Zhang, Z., Behringer, R. R., Crombrugghe, B., Postlethwait, J. H., Warman, M. L.,
Nakamura, T. and Akiyama, H
Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and
mediator subunit 25
Nat Commun, 2: 251, 2011.
・Suzuki, D., Yamada, A., Amano, T., Yasuhara, R., Kimura, A., Sakahara, M., Tsumaki, N.,
Takeda, S., Tamura, M., Nakamura, M., Wada, N., Nohno, T., Shiroishi, T., Aiba, A. and Kamijo, R
Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death
during limb development.
Dev. Biol., 335: 396-406, 2009.
・Iwai, T., Murai, J., Yoshikawa, H. and Tsumaki, N.
Smad7 Inhibits chondrocyte differentiation at multiple steps during endochondral bone
formation and down-regulates p38 MAPK pathways
J. Biol. Chem., 283: 27154-64, 2008.
・Murai, J., Ikegami, D., Okamoto, M., Yoshikawa, H. and Tsumaki, N
Insulation of the ubiquitous Rxrb promoter from the cartilage-specific adjacent gene,
Col11a2
J. Biol. Chem., 283: 27677-87, 2008.
・Nampei, A., Hashimoto, J., Koyanagi, J., Ono, T., Hashimoto, H., Tsumaki, N., Tomita, T.,
Sugamoto, K., Nishimoto, N., Ochi, T. and Yoshikawa, H.:
Characteristics of fracture and related factors in patients with rheumatoid arthritis.
Modern rheumatology / the Japan Rheumatism Association, 18: 170-6, 2008.
・Sugiki, T., Uyama, T., Toyoda, M., Morioka, H., Kume, S., Miyado, K., Matsumoto, K., Saito, H.,
Tsumaki, N., Takahashi, Y., Toyama, Y. and Umezawa, A.
Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9
chondroblasts.
J. Cell. Biochem., 100: 1240-54, 2007.
・Iwai, T., Harada, Y., Imura, K., Iwabuchi, S., Murai, J., Hiramatsu, K., Myoui, A., Yoshikawa, H.
and Tsumaki, N.
Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite
ceramicJ.
Bone Miner. Metab., 25: 392-9, 2007.
・Okamoto, M., Murai, J., Yoshikawa, H. and Tsumaki, N
Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts during bone
development.J.
Bone Miner. Res., 21: 1022-33, 2006.
・Hirao, M., Tamai, N., Tsumaki, N., Yoshikawa, H. and Myoui, A
Oxygen tension regulates chondrocyte differentiation and function during endochondral
ossification.
J. Biol. Chem., 281: 31079-92, 2006.
・Tsumaki, N., Kakiuchi, M., Sasaki, J., Ochi, T. and Yoshikawa, H
Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with
opening-wedge high tibial osteotomy by hemicallotasis
J. Bone Joint Surg. Am., 86-A: 2399-405, 2004.
・Horiki, M., Imamura, T., Okamoto, M., Hayashi, M., Murai, J., Myoui, A., Ochi, T., Miyazono, K.,
Yoshikawa, H. and Tsumaki, N
Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes
dwarfism with osteopenia.
J. Cell Biol., 165: 433-45, 2004.
・Katayama, R., Wakitani, S., Tsumaki, N., Morita, Y., Matsushita, I., Gejo, R. and Kimura, T
Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous
mesenchymal cells derived from bone marrow
Rheumatology (Oxford). 43: 980-5, 2004.
・Matsui, Y., Chansky, H. A., Barahmand-Pour, F., Zielinska-Kwiatkowska, A., Tsumaki, N.,
Myoui, A., Yoshikawa, H., Yang, L. and Eyre, D. R
COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma
fusion protein and wild-type ERG
J. Biol. Chem., 278: 11369-75, 2003
・Tsumaki, N., Nakase, T., Miyaji, T., Kakiuchi, M., Kimura, T., Ochi, T. and Yoshikawa, H
Bone morphogenetic protein signals are required for cartilage formation and differently
regulate joint development during skeletogenesis
J. Bone Miner. Res., 17: 898-906, 2002.
・Nakase, T., Sugamoto, K., Miyamoto, T., Tsumaki, N., Luyten, F. P., Inui, H., Myoui, A., Tomita, T.
and Yoshikawa, H
Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff.
Clin Orthop Relat Res: 140-5, 2002
・Matsui, Y., Araki, N., Tsuboi, H., Tsumaki, N., Nakata, K., Kawabata, H., Eyre, D. R. and
Yoshikawa, H
Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells
Clin Orthop Relat Res: 140-5, 2002
・Nakase, T., Ariga, K., Yonenobu, K., Tsumaki, N., Luyten, F. P., Mukai, Y., Sato, I. and
Yoshikawa, H
Activation and localization of cartilage-derived morphogenetic protein-1 at the site of
ossification of the ligamentum flavum
Eur. Spine J., 10: 289-94, 2001.
・Matsui, Y., Nakata, K., Araki, N., Ozono, K., Fujita, Y., Tsumaki, N., Kawabata, H., Yasui, N.,
Sekiguchi, K. and Yoshikawa, H
Alternative splicing of fibronectin transcripts in osteochondrogenic tumors
Anticancer Res., 21: 1103-6, 2001
・Matsui, Y., Yasui, N., Kawabata, H., Ozono, K., Nakata, K., Mizushima, T., Tsumaki, N.,
Kataoka, E., Fujita, Y. and Ochi, T
A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal
chondrodysplasia.
J. Hum. Genet., 45: 105-8, 2000.
・Liu, Y., Li, H., Tanaka, K., Tsumaki, N. and Yamada, Y.
Identification of an enhancer sequence within the first intron required for cartilage-specific
transcription of the alpha2(XI) collagen gene
J. Biol. Chem., 275: 12712-8, 2000.
・Tsumaki, N., Tanaka, K., Arikawa-Hirasawa, E., Nakase, T., Kimura, T., Thomas, J. T., Ochi, T.,
Luyten, F. P. and Yamada, Y.
Role of CDMP-1 in skeletal morphogenesis: promotion of mesenchymal cell recruitment
and chondrocyte differentiation.
J. Cell Biol., 144: 161-73, 1999
・Tsumaki, N., Kimura, T., Tanaka, K., Kimura, J. H., Ochi, T. and Yamada, Y.
Modular arrangement of cartilage- and neural tissue-specific cis-elements in the mouse
alpha2(XI) collagen promoter.
J. Biol. Chem., 273: 22861-4, 1998.
・Sugimoto, M., Kimura, T., Tsumaki, N., Matsui, Y., Nakata, K., Kawahata, H., Yasui, N.,
Kitamura, Y., Nomura, S. and Ochi, T
Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing
mouse
Cell Tissue Res., 292: 325-32, 1998.
・Matsui, Y., Yasui, N., Kimura, T., Tsumaki, N., Kawabata, H. and Ochi, T.
Genotype phenotype correlation in achondroplasia and hypochondroplasia.
J. Bone Joint Surg. Br., 80: 1052-6, 1998.
・Matsui, Y., Kimura, T., Tsumaki, N., Nakata, K., Yasui, N., Araki, N., Hashimoto, N., Uchida, A.
and Ochi, T
Splicing patterns of type XI collagen transcripts act as molecular markers for
osteochondrogenic tumors
Cancer Lett., 124: 143-8, 1998.
・Tsumaki, N., Kimura, T., Matsui, Y., Nakata, K. and Ochi, T.
Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI)
collagen gene expression in the embryonic mouse cartilage.
J. Cell Biol., 134: 1573-82, 1996.
・Tsumaki, N., Sugimoto, M., Nakata, K., Matsui, Y., Ochi, T. and Kimura, T.
Separable regulatory elements from pro-alpha 2 (XI) collagen gene drive distinct patterns
of cartilage-specific expression in transgenic mice.
Ann. N. Y. Acad. Sci., 785: 340-2, 1996.
・Matsui, Y., Kimura, T., Tsumaki, N., Yasui, N. and Ochi, T.
A recurrent 1992delCT mutation of the type X collagen gene in a Japanese patient with
Schmid metaphyseal chondrodysplasia.
Jpn. J. Hum. Genet., 41: 339-42, 1996.
・Kimura, T., Nakata, K., Tsumaki, N., Miyamoto, S., Matsui, Y., Ebara, S. and Ochi, T.
Progressive degeneration of articular cartilage and intervertebral discs. An experimental
study in transgenic mice bearing a type IX collagen mutation
Int. Orthop., 20: 177-81, 1996
・Iwasaki, M., Okada, K., Tsumaki, N., Obata, H., Shirasaki, N. and Oka, S
Cervical spondylotic radiculopathy involving two adjacent nerve roots. Anterior
decompression through a single level intervertebral approach.
Int. Orthop., 20: 137-41, 1996.
・Tsumaki, N. and Kimura, T.
Differential expression of an acidic domain in the amino-terminal propeptide of mouse
pro-alpha 2(XI) collagen by complex alternative splicing.
J. Biol. Chem., 270: 2372-8, 1995.